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KMID : 0545120070170111894
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Journal of Microbiology and Biotechnology
2007 Volume.17 No. 11 p.1894 ~ p.1897
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Changes in Aurantio-Obtusin and Glucoaurantio-Obtusin Content in Cassiae Semen via Treatment with a Crude Enzyme Extract from Aspergillus usamii
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Hur Jong-Moon
Kwon Soon-Ho
So Jae-Hyun
Jun Mi-Ra
Kang Young-Hwa
Lee Yu-Mi
Lee Kyung-Bok
Rhee In-Koo
Lee Moon-Soon
Song Kyung-Sik
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Abstract
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Cassiae Semen (seeds of Cassia tora) showed a remarkably different HPLC chromatogram after being treated with a crude enzyme extract from Aspergillus usamii. Increased and decreased compounds were identified as aurantio-obtusin and glucoaurantio-obtusin, respectively. The aurantio-obtusin content reached its maximum level (133.58±0.39 μg/mg extract) after being incubated for 50 min at 37oC, whereas the inactivated crude enzyme-treated control remained unchanged (54.13±1.33 μg/mg). On the other hand, the glucoaurantioobtusin content decreased by less than one-third (51.09±1.63 μg/ mg) of the untreated control (143.19±2.12 μg/mg), suggesting that an increase in aurantio-obtusin content originated from the enzymatic cleavage of its glucoside glucoaurantio-obtusin
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KEYWORD
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Cassiae Semen, aurantio-obtusin, glucoaurantioobtusin, Aspergillus usamii, processes, enzyme treatment
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